Figure 4From: The disarrayed mutation results in cell cycle and neurogenesis defects during retinal development in zebrafishInitiation of retinal neurogenesis in disarrayed eyes. (A) In situ analysis of ath5 expression during retinogenesis in wild-type (i, iii, v, vii) and disarrayed eyes (ii, iv, vi, viii). (B) BrdU pulse-labeling (red) in wild-type (upper) or disarrayed (lower) embryos carrying the ath5:gfp transgene (green). Embryos were injected with BrdU at 36 hpf and fixed at 42 hpf. Note that in both genotypes, ath5:GFP expression in central retinal cells is post-S-phase. (C) Developmental time (hours post fertilization) for the initiation of huc:GFP expression. The initiation of GFP expression was recorded for all embryos at 0.5 hr intervals in a clutch from an in-cross of disarrayed heterozygous parents (one circle represents one embryo; mutant (black circles) and wild-type (white circles)). Note that all embryos initiate huc:GFP expression within the same window of development. Results from one representative experiment (n = 16 mutant and n = 45 wild-type embryos from one clutch of embryos).Back to article page