Figure 3

Proliferation defects in disarrayed retinas. (A) One hour labeling with BrdU (red) at 48 hpf (i, ii), 72 hpf (iii, iv) and 96 hpf (v, vi) in wild-type and disarrayed embryos. Wild-type proliferative cells are restricted to the marginal zone by 72 hpf, whereas the disarrayed retinal progenitors do not restrict to the marginal zone until 96 hpf. (B) Quantization of the one hour BrdU-injections presented as the percentage of BrdU-positive cells divided by total cell number at 48 hpf, 72 hpf and 96 hpf. ** p ≤ 0.01 (Student's t-test). Grey bars, wild-type; black bars, disarrayed. Three central retinal sections were counted from three independent embryos for each time point.