Table 1 Summary of all characterization experiments performed along with their outcome.

ENDOGENIC DIFFERENTIATION

Cytological staining

PAS

++

-

NT

NT

Immunofluorescence

AFP, HNF3β, ALB, CK18, HNF1α, C/EBPα, MRP2

-

-

+++

++

Functional analysis

ALB secretion

-

-

+++

++

Ureogenesis

-

-

+++

++

EROD activity

-

-

+++

++

Inducible EROD activity

-

-

+++

++

PROD activity

+

-

+++

++

Inducible PROD activity

-

-

+++

++