Figure 9
EROD-activities and responsiveness to 2 μM MC in sequentially (+/-1 μM TSA) or cocktail (+/-1 μM TSA)-exposed hMSC. MC was added daily, starting on day 18. Gray area represents EROD-activity measured in 4 hours-cultured adult hepatocytes, not treated with TSA. The graph is representative for 5 separate experiments, each performed in duplicate. MC, 3-methylcholantrene. *, **: EROD-activity of sequentially TSA- and cocktail TSA-exposed hMSC is significantly higher than of sequentially- and cocktail-exposed hMSC with p < 0.05 and p < 0.01, respectively (Student's t-test). $,$$: MC significantly induced EROD-activity of sequentially TSA- and cocktail TSA-exposed hMSC with p < 0.05 and p < 0.01, respectively(Student's t-test). °°°: CYP1A1/2-inducibility significantly differs among sequentially TSA- and cocktail TSA-exposed hMSC with p < 0.001 (Student's t-test).