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Table 4 Fertilization defects associated with mfr mutations

From: Complex regulation and multiple developmental functions of misfire, the Drosophila melanogasterferlin gene

Male Genotype*

# of Eggs Examined

Percent Inseminated Eggs∞

Sperm Head Condensed‡

Subsequent Development‡ (number of eggs)

mfrZ0695/Df

40

86%

100%

 

mfrZ6248/Df

31

85%

100%

 

mfrZ2942/Df

30

100%

93%

Sperm decondensation (1); Pronuclear apposition (1)

mfrZ3281/Df

30

92%

92%

Sperm decondensation (1); Pronuclear apposition (1)

mfrZ2713/Df

29

100%

90%

Pronuclear apposition (3)

  1. * males express dj-GFP as sperm tail marker; Df is Df(3L)h-i22
  2. ∞ percentage of inseminated eggs relative to a control cross of dj-GFP males mated to wild-type females; DJ-GFP was detected in 87% of the eggs
  3. ‡ sperm chromatin visualized by DAPI staining
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