Figure 6
Sperm produced by mfr males retain the CD2 membrane marker during fertilization. For these confocal images, sperm produced by mfrZ0695 males were assayed (A) in the testis or (B) after entry into the egg. CD2 (green) was detected by immunolocalization and nuclei (blue) were detected by DAPI staining. Merged images show that in the egg, CD2 persists around the head and tail, indicating failure in sperm PMBD. Scale bars are approximately ten microns.