Figure 3

S100B and EGFP are co-expressed in the radial glial scaffold of the cerebellar plates (E14.5 and E16.5). A: lower power view of the lateral portion (future hemisphere) of the E14.5 cerebellum plate. B: higher power view of the boxed area in (A), illustrating localization of cell somata in the VZ and long radial processes traversing a CTZ filled with post-mitotic β3-tubulin+ neuron precursors (inset). C: zooming on the VZ area reveals a high level of S100B/EGFP colocalisation at the single cell level. D: As expected for radial glial cells, EGFP+ cells present in the VZ express BLBP. E: at E16.5, and in addition to radial glial cells and CPe cells, Sox9 expression is maintained in isolated cells emigrating from the VZ (arrowheads).