Table 2 Primer sequences and parameters of standard curves for the selected genes used in the experiment
Gene | Forward Reverse | Slope (m) | Intercept (b) | R2 |
|---|---|---|---|---|
Actb | ATGAGCTGCCTGACGGCCAGGTCATC TGGTACCACCAGACAGCACTGTGTTG | -2.97 | 22.16 | 0.99 |
Gapdh | TGACGTGCCGCCTGGAGAAA AGTGTAGCCCAAGATGCCCTTCAG | -3.49 | 21.69 | 0.98 |
Hprt1 | GCTTGCTGGTGAAAAGGACCTCTCGAAG CCCTGAAGTACTCATTATAGTCAAGGGCAT | -3.57 | 23.02 | 0.97 |
H2afz | ACAGCGCAGCCATCCTGGAGTA TTCCCGATCAGCGATTTGTGGA | -3.81 | 21.20 | 0.99 |
Ubc | CGTCGAGCCCAGTGTTACCACCAAGAAGG CCCCCATCACACCCAAGAACAAGCACAAG | -3.67 | 23.32 | 0.97 |
Ppia | CGCGTCTCCTTCGAGCTGTTTG TGTAAAGTCACCACCCTGGCACAT | -3.71 | 20.84 | 0.99 |
Eef1e1 | GCGGAGTTGAGGCTGCTGGAGA AGACTCGGGCCATTGTTTGTCTG | -2.96 | 26.95 | 0.96 |