Table 2 Primer sequences and parameters of standard curves for the selected genes used in the experiment
| Gene | Forward Reverse | Slope (m) | Intercept (b) | R2 |
|---|---|---|---|---|
| Actb |
ATGAGCTGCCTGACGGCCAGGTCATC TGGTACCACCAGACAGCACTGTGTTG | -2.97 | 22.16 | 0.99 |
| Gapdh |
TGACGTGCCGCCTGGAGAAA AGTGTAGCCCAAGATGCCCTTCAG | -3.49 | 21.69 | 0.98 |
| Hprt1 |
GCTTGCTGGTGAAAAGGACCTCTCGAAG CCCTGAAGTACTCATTATAGTCAAGGGCAT | -3.57 | 23.02 | 0.97 |
| H2afz |
ACAGCGCAGCCATCCTGGAGTA TTCCCGATCAGCGATTTGTGGA | -3.81 | 21.20 | 0.99 |
| Ubc |
CGTCGAGCCCAGTGTTACCACCAAGAAGG CCCCCATCACACCCAAGAACAAGCACAAG | -3.67 | 23.32 | 0.97 |
| Ppia |
CGCGTCTCCTTCGAGCTGTTTG TGTAAAGTCACCACCCTGGCACAT | -3.71 | 20.84 | 0.99 |
| Eef1e1 |
GCGGAGTTGAGGCTGCTGGAGA AGACTCGGGCCATTGTTTGTCTG | -2.96 | 26.95 | 0.96 |