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Table 2 Antibodies used for immunofluorescent immunohistochemistry

From: Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis

Antibody Dilution Blocking Serum Detection
DAZL+VASA DAZL 1/500+VASA 1/300 goat GAMB+Streptavidin-546 (DAZL)
GAR-488 (VASA)
VASA + OCT 4 VASA 1/300+OCT 41/200 chicken CARB+Streptavidin-488 (VASA)
CAGP + Tyr-Cy3(OCT4)
DAZL + OCT 4 DAZL 1/500 + OCT 4 1/200 rabbit RAMB+Streptavidin 546 (DAZL)
RAGP+TyrCy3 (OCT4)
  1. VASA, ab13840, Abcam, Cambride UK
  2. OCT4, Sc8629, Santa Cruz, CA, USA
  3. DAZL MCA 2336, Serotec, Oxford, UK
  4. GAMB = goat anti-mouse biotinylated secondary antibody (DAKO, UK) diluted 1/500
  5. Streptavidin-546 (Invitrogen, UK) diluted 1/200
  6. GAR-488 = goat anti-rabbit-488 (Invitrogen, UK) diluted1/200
  7. CARB = chicken anti-rabbit biotinylated (Santa Cruz, CA, USA) diluted 1/200
  8. RAMB = rabbit anti-mouse biotinylated (DAKO, UK) diluted 1/500
  9. CAGP = chicken anti-goat peroxidase (Santa Cruz, CA, USA) diluted 1/200
  10. Tyr-Cy3 = Tyramide Cy3 (Molecular Probes, UK) diluted 1/5.