Skip to main content

Table 2 Antibodies used for immunofluorescent immunohistochemistry

From: Conserved and divergent patterns of expression of DAZL, VASA and OCT4 in the germ cells of the human fetal ovary and testis

Antibody

Dilution

Blocking Serum

Detection

DAZL+VASA

DAZL 1/500+VASA 1/300

goat

GAMB+Streptavidin-546 (DAZL)

GAR-488 (VASA)

VASA + OCT 4

VASA 1/300+OCT 41/200

chicken

CARB+Streptavidin-488 (VASA)

CAGP + Tyr-Cy3(OCT4)

DAZL + OCT 4

DAZL 1/500 + OCT 4 1/200

rabbit

RAMB+Streptavidin 546 (DAZL)

RAGP+TyrCy3 (OCT4)

  1. VASA, ab13840, Abcam, Cambride UK
  2. OCT4, Sc8629, Santa Cruz, CA, USA
  3. DAZL MCA 2336, Serotec, Oxford, UK
  4. GAMB = goat anti-mouse biotinylated secondary antibody (DAKO, UK) diluted 1/500
  5. Streptavidin-546 (Invitrogen, UK) diluted 1/200
  6. GAR-488 = goat anti-rabbit-488 (Invitrogen, UK) diluted1/200
  7. CARB = chicken anti-rabbit biotinylated (Santa Cruz, CA, USA) diluted 1/200
  8. RAMB = rabbit anti-mouse biotinylated (DAKO, UK) diluted 1/500
  9. CAGP = chicken anti-goat peroxidase (Santa Cruz, CA, USA) diluted 1/200
  10. Tyr-Cy3 = Tyramide Cy3 (Molecular Probes, UK) diluted 1/5.