Figure 3

Areas of co-expression between nr4a2a/b , pitx3 and lmx1b.1 in the posterior tuberculum. (A-D") Confocal z-projections of double whole mount FISH at 48 hpf show overlapping expression domains for nr4a2a/b, pitx3 and lmx1b.1. The projections encompass the focal planes through the ventral diencephalon. The antisense probes used for the FISH are indicated on the top of each panel. (A-A") 28 μm projection; (B-B") 19 μm projection; (C-C") 16 μm projection; (D-D") 13 μm projection. Anterior is to the left. Scale bars: 50 μm. Abbreviation: PT, posterior tuberculum.