Figure 7
From: Incudomalleal joint formation: the roles of apoptosis, migration and downregulation
Downregulation of cartilage fate in the incudomalleal region. (A) Collagen type II immunohistochemistry of the middle ear ossicles at E14.5. The ossicles appear positive (brown) while the presumptive joint is negative by this stage. (B) In situ hybridisation showing the expression of Gdf5 in the joint region of the middle ear at E14.5. (C) Dissected middle ear ossicles after wholemount staining for LacZ at E14.5. Strong expression of LacZ is visible in between the ossicles, which appear united (arrow). (D, E) Type II collagen lacZ reporter mouse. Collagen type II immunohistochemistry of the middle ear at E14.5 (brown) and lacZ staining (blue). β gal positive cells are present in the joint region and ossicles, while the Type II collagen protein is only present in the ossicles. Arrows point to joint region. Scale bar in (A) = 100 μm. Scale bars in (C) = 175 μm. Scale bar in (D) = 200 μm. Scale bar in (E) = 70 μm. M = malleus. I = incus.