Skip to main content

Table 1 Quantity of p66Shc, p53 and H2a mRNA transcripts in early cleaving, late cleaving, 2–4 cell arrested embryos and bovine blastocysts

From: The oxidative stress adaptor p66Shc is required for permanent embryo arrest in vitro

 

Quantity of mRNA transcripts/embryo (pg)

 

Early cleaving embryos (28 hpi)

Late cleaving embryos (29 to 48 hpi)

2–4 cell arrested embryos

Blastocysts

p66Shc

1.20 ± 0.30 × 10-5 a

2.80 ± 0.32 × 10-5 b

4.02 ± 0.21 × 10-5 c

0.50 ± 0.07 × 10-5 d

H2a

0.53 ± 0.09 × 10-3 a'

0.43 ± 0.12 × 10-3 a'

0.46 ± 0.13 × 10-3 a'

0.43 ± 0.13 × 10-3 a'

p53

0.95 ± 0.13 × 10-6 a"

1.01 ± 0.15 × 10-6 a"

0.81 ± 0.22 × 10-6 a"

1.70 ± 0.50 × 10-6 a"

  1. Values represent mean ± SEM
  2. Different superscripts differ significantly (a,bP < 0.005; a,cP < 0.0001; a,dP < 0.001)