Figure 5

Real Time PCR quantification of p66Shc mRNA levels at the blastocyst stage following RNAi. Real Time PCR analysis of p66Shc (A) and H2a (B) mRNA levels in day 8–9 blastocysts obtained following control IVF, microinjection of the vehicle and microinjection of 12,000 p66Shc short hairpin shRNAi molecules. No significant differences (P = 0.314) in the p66Shc mRNA levels were measured (A). The quantification of the histone H2a mRNA levels was used as a control, observing no significant differences (P = 0.312) (B). The experiments were conducted on three different pools of 80–100 embryos (n = 3) and replicated three times (r = 3) for each sample. The data were normalized for the content in one blastocyst.