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Table 1 Apoptosis during tailbud eversion. Number of cells labeled using the TUNEL assay in 18–19 som (A) and 22–23 som (B) embryos is shown as mean ± SD. (1, 2, 3) Tail regions in which the TUNEL-positive cells were counted (refer to figure 6 for illustration). 0.4 ng of vangl2 MO was injected into each embryo where specified

From: Cadherin-mediated adhesion regulates posterior body formation

A

Genotype

 

18–19 somites

   

1

2

3

WT

 

(n = 4)

2.75 ± 1.26

2.75 ± 2.22

1.75 ± 2.22

N-cad m117

 

(n = 3)

11.00 ± 3.46

10.33 ± 2.31

7.33 ± 1.53

WT w/vangl2 MO

 

(n = 3)

6.67 ± 5.51

9.00 ± 6.93

10.67 ± 10.07

N-cad p79emcf

 

(n = 3)

6.33 ± 0.58

13.33 ± 1.15

1.33 ± 1.53

N-cadp79emcfw/vangl2 MO

 

(n = 2)

14.00 ± 8.48

6.50 ± 3.54

3.00 ± 2.83

B

Genotype

 

22–23 somites

   

1

2

3

WT

 

(n = 4)

3.00 ± 3.56

2.00 ± 2.83

3.25 ± 2.36

N-cad m117

 

(n = 7)

3.14 ± 1.77

5.57 ± 3.21

0.29 ± 0.49

WT w/vangl2 MO

 

(n = 7)

1.29 ± 2.56

0.71 ± 1.25

1.00 ± 1.00

N-cad p79emcf

 

(n = 8)

2.13 ± 2.03

1.63 ± 1.60

0.50 ± 0.53

N-cadp79emcfw/vangl2 MO

 

(n = 7)

1.86 ± 1.57

0.86 ± 0.69

1.71 ± 1.60