Figure 5

Rux is necessary to maintain G1 arrest in the posterior of the NPR. (A-B) rux⁸ clones (absence of β-galactosidase in green) stained for BrdU (A) and CycB (B) in red. Both ectopic BrdU incorporation and CycB accumulation lie predominantly in the posterior part of the NPR, although occasional BrdU positive cells are also seen more anteriorly. (C-D) PCNA-GFP reporter expression (green in C and D, and white in C' and D') in discs with rux⁸ clones (absence of red in C) and CycA overexpression clones (marked by presence of β-galactosidase (red in D). The PCNA-GFP reporter is slightly activated in the rux clone in the NPR (arrow in C') and in cells overexpressing CycA in the NPR (arrow in D'). (F-G) rux-lacZ transgene expression (red) in a WT disc (F) and in a disc harbouring smo³ tkv^a12 double mutant clones. The transgene is expressed in the NPR, the position of which is localised by the expression of Ato (blue in F), and its levels decreases just posterior to the MF. The levels of rux-lacZ increase in the double mutant cells for smo and tkv (marked by absence of green in G). The bar indicates the width of the NPR.