Figure 2

Effect of overexpression of cyclins in the NPR. All panels except B show BrdU staining (red) of eye disc harbouring different overexpression clones marked with GFP (green). (A) The overexpression of CycE is able to induce ectopic BrdU incorporation with a high efficiency in the anterior part of the NPR, but less so the posterior, where only a few cells enter S phase. (B) The PCNA-GFP reporter (green) is active (arrows) in the CycE overexpression clones (marked by presence of β-gal in red) within the NPR. (C) In dE2F1 overexpression clones there is BrdU incorporation only in the anterior part of the NPR. (D) The overexpression of CycE and dE2F1 can induce incorporation of BrdU in anterior and posterior cells of the NPR. (E) CycA overexpressing cells incorporate BrdU in any part of the NPR, but with a higher efficiency in the posterior part, where most cells are BrdU positive. (F) Simultaneous overexpression of CycE and CycA induces a high proportion of BrdU incorporation in any part of the NPR.