Figure 7
Developmental expression of AmphiHtt in B. floridae neurulae. In the whole mounts, anterior is on the left; the cross sections are counterstained in pink and viewed from the posterior end of the animal. Scale lines for whole mounts = 50 μm and for sections = 25 μm. A. Side view of 11-hour neurula with AmphiHtt expression in the most anterior neural plate (arrow), and in some cells of the neural folds (arrowheads). B, C. Cross sections through levels b and c in A. Transcripts are visible in the most anterior tip (arrows) and at the edges (arrowheads) of the neural plate. D. Side view of 15-hour neurula with AmphiHtt expression in the cerebral vesicle and the most anterior two-thirds of the neural tube. Some labelled cells are also visible in Hatschek's left diverticulum (arrowhead) and in cells of the neuroenteric canal (arrows). E-J. Cross sections through levels e-j in D. Strong neural expression was found in the cerebral vesicle (E-G) and ventrolateral neurons of the neural tube (H-J). AmphiHtt is also expressed in some cells of Hatschek's left diverticulum (arrowhead) (G). K. Cross sections through level k in D showing expression in the endoderm of the neuroenteric canal (arrows). L. Side view of 18-hour neurula: AmphiHtt expression is mainly localised at somite level and in the neural tube. M. Cross-sections through level m in L show details of the transcript localisation in a pair of ventrolateral neurons in the neural tube. N. High-resolution images of the preceding specimens throughout somites 3–10. O-R Cross-sections through levels o-r in N showing AmphiHtt expression in groups of cells located dorsolaterally in the somites. The arrow in P indicates the primary pigment spot in the nerve cord. S. Frontal section of 18-hour neurula showing two labelled cell bodies located near the somite boundaries. Abbreviations: n, notochord; asterisks, Haschek's left diverticulum; nc, neuroenteric canal.