Skip to main content

Advertisement

Table 4 Expression of Tarama* rescues mesoderm formation and neural tube closure

From: Nodal signaling is required for closure of the anterior neural tube in zebrafish

   Pineal morphology
Embryo MRNA Round Expanded Divided
MZoepm134 None    
hg-; nc-   0 6 19
hg-; nc+   0 0 0
hg+; nc-   0 0 0
hg+; nc+   0 0 0
MZoepm134 GFP    
hg-; nc-   0 5 30
hg-; nc+   0 0 0
hg+; nc-   0 0 0
hg+; nc+   0 0 0
MZoepm134 Tarama*    
hg-; nc-   0 8 15
hg-; nc+   0 1 2
hg+; nc-   5 7 7
hg+; nc+   19 5 2
WT Various    
hg-; nc-   0 0 0
hg-; nc+   0 0 0
hg+; nc-   0 0 0
hg+; nc+   33 0 0
  1. Embryos were fixed at approximately 1 dpf and processed for whole mount in situ hybridization with an antisense probes to otx5, ctsl1b, and col2a1a. *WT embryos are a mixture of uninjected embryos, embryos injected with 2.4 pg of mGFP mRNA and embryos injected with 2.4 pg Taram-A* mRNA. MZoep embryos were injected with between 0.15 and 2.4 pg of Taram-A* mRNA, with the results for all doses grouped together or with 2.4 pg of mGFP mRNA. Whether hatching gland (hg) and notochord (nc) cells are present (+) or absent (-) is indicated.