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Table 1 Embryos that lack Nodal signaling or N-cad expression have defects in the convergence of pineal precursors

From: Nodal signaling is required for closure of the anterior neural tube in zebrafish

  Pineal morphology
Embryo Normal Expanded Divided
sqtcz35/+ X sqtcz35/+    
   cyclopic eye 90 45 61
   normal eyes* 814 3 1
cyc m294 140 0 0
WT siblings 343 0 0
Zoepm134 83 0 0
WT siblings 157 0 0
MZoepm134 0 5 117
pac p79m 58 18 10
WT siblings 191 0 0
glo m117 13 17 28
WT siblings 85 0 0
  1. Embryos were fixed at 1–3 dpf (for sqt), 1 dpf (for cyc, Zoep, MZoep, and pac), or 2–4 dpf (for glo), and then processed for whole mount in situ hybridization with a pineal specific probe (otx5, flh, gnat1, gnat2) or antibody staining with the pineal photoreceptor specific antibody zpr1.
  2. *Embryos with two normal eyes could be homozygous WT, heterozygous for sqtcz35, or homozygous for sqtcz35.