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Table 1 Embryos that lack Nodal signaling or N-cad expression have defects in the convergence of pineal precursors

From: Nodal signaling is required for closure of the anterior neural tube in zebrafish

 

Pineal morphology

Embryo

Normal

Expanded

Divided

sqtcz35/+ X sqtcz35/+

   

   cyclopic eye

90

45

61

   normal eyes*

814

3

1

cyc m294

140

0

0

WT siblings

343

0

0

Zoepm134

83

0

0

WT siblings

157

0

0

MZoepm134

0

5

117

pac p79m

58

18

10

WT siblings

191

0

0

glo m117

13

17

28

WT siblings

85

0

0

  1. Embryos were fixed at 1–3 dpf (for sqt), 1 dpf (for cyc, Zoep, MZoep, and pac), or 2–4 dpf (for glo), and then processed for whole mount in situ hybridization with a pineal specific probe (otx5, flh, gnat1, gnat2) or antibody staining with the pineal photoreceptor specific antibody zpr1.
  2. *Embryos with two normal eyes could be homozygous WT, heterozygous for sqtcz35, or homozygous for sqtcz35.
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BMC Developmental Biology

ISSN: 1471-213X

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