Table 2 primers sequences
gene (transcript variant) | primer | sequence | Genbank accession |
|---|---|---|---|
luciferase | fwd | TCATTCTTCGCCAAAAGCACTCTG | Â |
| Â | rev | AGCCCATATCCTTGTCGTATCCC | Â |
18S | fwd | CGGACCAGAGCGAAAGCATTTG | DQ222453 |
| Â | rev | GAATAACGCCGCCGCATCG | Â |
ACTB | fwd1 | CGTGACATTAAGGAGAAGCTGTGC | NM_173979 |
| Â | fwd2 | GCGTGACATCAAGGAGAAGC | Â |
| Â | rev1 | CTCAGGAGGAGCAATGATCTTGAT | Â |
| Â | rev2 | TGGAAGGTGGACAGGGAGGC | Â |
MATER | fwd | GCTGGAGGCGTGTGGACTG | NM_001007814 |
| Â | rev | GGTCTGTAGATTAGAGGTGGGATGC | Â |
NALP9 | fwd | GCGGCGGTGCTGTGTGAAG | NM_001024664 |
| Â | rev | CTGCGTCTGCCCTCGTCATC | Â |
ZAR1 | fwd | TGCCGAACATGCCAGAAG | NM_001076203 |
| Â | rev | TCACAGGATAGGCGTTTGC | Â |
MELK | fwd1 | CCTTGATGAAGATTGTGTAACGGAAC | EF446902 |
| Â | fwd2 | CCAGGTAGCAAAAGGAAAGGC | Â |
| Â | rev1 | GGAAGAAAGAAGCCTTAAACGAACC | Â |
| Â | rev2 | CTTTGGAAGAACAGAGCATTATTTC | Â |
BCAR4 (v1+v2) | fwd1 | GAAGGGTGTTTGCTGATTTCTGTTAAG | EF446907 |
| Â | fwd2 | CCTGAAGGGTGTTTGCTGAT | Â |
| Â | rev1 | CATTGTTGTTACCAGGGCGAAGG | Â |
| Â | rev2 | CCAGGTCCACTGACTGTTAGC | Â |
BCAR4 (v1) | fwd | TCTTGTCCATCGTCCTGTCCTG | EF446906 |
| Â | rev | GTAGACCACACCATTACATCAGAGG | Â |
RNF18 | fwd1 | TGCTGTGCTTGTTCTGCTCTC | EF446903 |
| Â | fwd2 | GCTTCTTTTGCTCTCTCTCATCTCCTC | Â |
| Â | rev1 | GCTCGTATCATCACCAGTCGTAG | Â |
| Â | rev2 | AATGGTGGCAGGGTCTGTGAA | Â |
STELLA (v1) | fwd | TAGGACTACGCCCATTCACC | EF446904 |
| Â | rev1 | TGCTGTAGGCTCAAACTGCTC | Â |
| Â | rev2 | GGGTCCAGGTTGGGTTATCT | Â |
STELLA (v2) | fwd1 | GCGGGGATGGCTACTCTTC | EF446905 |
| Â | fwd2 | TCTTCATCCCCTACAAAAGCA | Â |
| Â | rev1 | TGCTGTAGGCTCAAACTGCTC | Â |
| Â | rev2 | GGGTCCAGGTTGGGTTATCT | Â |
CCNB1 | fwd | TGGGTCGGCCTCTACCTTTGCACTTC | NM_001045872 |
| Â | rev | CGATGTGGCATACTTGTTCTTGATAGTCA | Â |
CDC2 | fwd | ATGGCTTGGATCTGCTCTCG | NM_174016 |
| Â | rev | CATTAAAGTACGGATGATTCAGTGC | Â |
PRDX1 | fwd | TCAAGCCTGATGTCCAGAAGAGC | NM_174431 |
| Â | rev | CCGTCCTGTCCCACACCAC | Â |
PRDX2 | fwd | GATTATGGCGTGCTGAAGGAAGATG | NM_174763 |
| Â | rev | GAGCGTCCCACAGGCAAGTC | Â |
ID2 | fwd | CAGTCCAGTGAGGTCCGTTAGG | AW464352 |
| Â | rev | GCAGGCTCATCGGGTCGTC | Â |
ID3 | fwd | TGACGACATGAACCACTGCTACTC | BM106920 |
| Â | rev | GCTGTCTGGATGGGAAGATGCG | Â |
AURKA | fwd | TCGGGAGGACTTGGTTTCTT | DQ334808 |
| Â | rev | TGTGCTTGTGAAGGAACACG | Â |