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Figure 1 | BMC Developmental Biology

Figure 1

From: Differential regulation of abundance and deadenylation of maternal transcripts during bovine oocyte maturation in vitro and in vivo

Figure 1

Characterization of oocyte specific transcripts. (A) Expression pattern of MELK, RNF18, BCAR4 and STELLA in bovine somatic and gonadic tissues by RT-PCR. Tissues are indicated above the lanes: brain (Br), heart (He), pituitary gland (Pt), intestine (In), liver (Li), lung (Lu), muscle (Mu), spleen (Sp), uterus (Ut), testis (Te), Ovary (Ov), oocyte (Oo); no PCR substrate as negative control (-). RT-PCR for ACTB is shown as a positive control. An estimated 500-fold higher amount of substrate was used in PCR for somatic tissues and gonads as compared to the oocyte. (B) Schematic representation of STELLA transcript variants generated by alternative splicing. (C) Schematic representation of BCAR4 variants generated by alternative use of two polyadenylation signals (AAUAAA).

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BMC Developmental Biology

ISSN: 1471-213X

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