Figure 8

Tyrosine phosphorylated Shc after stimulation with IGF-II. Protein lysates from cells treated with IGF-II for 20 mins were analysed by Western blot. A: Shows a representative western blot analysis for total Shc. Protein lysates were immunoprecipiated with an anti-Shc antibody as described in the materials and methods and the precipitate was run on a Western blot and stained with the antibody p-Tyr and a representative Western blot is shown in A. B: shows the graph plotting the densometry, after correcting for total Shc. A statistical significant difference was demonstrated for p46 with MDF showing a significant increase after treatment (*p = 0.03) and the MDF treated group was statistically greater than the FDF treated group (**p = 0.004). n = 5 FDF and n = 5 MDF (closed bar 46 kDa, open bar 52 kDa, and hashed bar 66 kDa). Error bars = SEM.