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Figure 6 | BMC Developmental Biology

Figure 6

From: Dermal fibroblasts derived from fetal and postnatal humans exhibit distinct responses to insulin like growth factors

Figure 6

Tyrosine phosphorylated proteins after stimulation with IGF-II. A: Representative Western blot. Protein lysates from cells treated with IGF-II for 20 mins were analysed by Western blot using a specific antibody against tyrosine phosphorylated proteins. B: To ensure equal loading used the MemCode Reversibleâ„¢ protein stain (Pierce Biotechnology) C: The graph represents the mean change in tyrosine phosphorylation after ensuring for equal loading following stimulation with IGF-II for FDF (n = 5 open bar un-stimulated; lined bar represents stimulated lysates) and MDF (n = 5 closed bar represents un-stimulated lysates; thick lines represents stimulated lysates). Error bars = SEM. A statistical decrease in FDF (p = 0.048) was identified for a band at 50 kDa following stimulation with IGF-II. FDF showed a statistical increase when comparing un-stimulated cells with stimulated cells at 100 kDa (p = 0.029) and a further statistical increase was demonstrated between FDF and MDF when cells had been stimulated with IGF-II at for a band at 100 kDa (p = 0.049).

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