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Figure 3 | BMC Developmental Biology

Figure 3

From: Dermal fibroblasts derived from fetal and postnatal humans exhibit distinct responses to insulin like growth factors

Figure 3

Collagen production in response to IGF-II. Radio-labelled proline uptake assay demonstrating modulation of protein synthesis by IGF-II. Panel A and B depict IGF-II elicited changes in collagen and non-collagen synthesis respectively, by FDF (n = 3, open squares) and MDF (n = 3, closed diamonds). All data is represented as cpm/105 cells (mean ± sem). Panel C illustrates changes in relative collagen synthesis between MDF and FDF in response to IGF-II; data represented as % (mean ± sem). * p = < 0.05. Collagen synthesis was significantly higher for FDF over untreated control at 100 ng/ml IGF-II. There was no significant change in non-collagen synthesis for either cell type. Student t-tests demonstrated that the %RCS produced by FDF with 50 ng/ml IGF-II was significantly higher than that produced by PDF (*p = 0.016). ANOVA analysis showed that both collagen count data and % RCS data from FDF are highly significantly different (p = 0.003 and 0.010 respectively) from PDF cell strain data.

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