Regulated Otx2-GFP nuclear localization in adult retina. Confocal microscopy analysis. a, b. Direct visualisation of GFP fluorescence (green) and propidium iodide staining of DNA (red) on 10 μm thin section of Otx2+/OG(a) and control Otx2+/+(b) adult retina. c-f". Localisation of Otx2-GFP (direct visualisation of GFP fluorescence – green, c,d,e',e"), immunofluorescent staining of nuclear envelope (lamin B – red, c,d,e,e", f, f") and immunofluorescent staining of Otx2 (green – f', f") on 10 μm thin section of Otx2+/OGand wild type adult retina showing retinal pigment epithelium cells (RPE, c), outer cells of the inner nuclear layer (INL, d) and photoreceptor cells of the outer nuclear layer (ONL, e-f"). Image e" and f" are overlays of images e and e' and of images f and f' respectively. Scale bars: 10 μm.