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Figure 5 | BMC Developmental Biology

Figure 5

From: The Drosophila Perlecan gene trol regulates multiple signaling pathways in different developmental contexts

Figure 5

Mutations in trol decrease circulating plasmatocyte number and pnt expression. A) Quantification of circulating plasmatocytes in trolb22 and trol7 mutants compared to controls. Each sample consisted of hemolymph pooled from three third instar larvae. Five squares were counted for each sample. Each genotype was analyzed in triplicate. B) Brightfield image of plasmatocytes from Trol-GFP stock. C) Fluorescence image of plasmatocytes from Trol-GFP stock demonstrating presence of Trol on plasmatocytes. D) Expression of the VEGF/PDGF response gene pnt in trolb22 and trol7 mutant hemocytes compared to sibling controls by qRT-PCR. Samples of hemolymph from three third instar larvae of each genotype were pooled, RNA extracted, amplified and analyzed. All reactions were carried out in triplicate at three different template concentrations to ensure amplification was in the linear range. β-actin was used as an internal normalization control.

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