Table 4 Transplantation of chch-EnR cells requires nodal signaling for cell movements and fate changes
From: Churchill regulates cell movement and mesoderm specification by repressing Nodal signaling
| Â | Â | Â | Location of cells at shield | Location of Cells at 24 hpf | |||||
|---|---|---|---|---|---|---|---|---|---|
Donor cells | Host embryos | n | Animal pole | Germ ring | Neural | Epidermis | Somites | Notochord | Yolk (Superficial) |
LacZ | Wild-type | 22 | 22 | 0 | 19 | 3 | 0 | 0 | 0 |
LacZ | Wild-type + SB431542 | 25 | 25 | 0 | 25 | 0 | 0 | 0 | 0 |
chch-EnR | Wild-type | 32 | 19 | 13 | 11 | 1 | 17 | 2 | 0 |
chch-EnR | Wild-type + SB431542 | 32 | 32 | 0 | 24 | 0 | 0 | 0 | 8 |
MZoep-LacZ | Wild-type | 36 | 35 | 1 | 29 | 7 | 0 | 0 | 0 |
Mzoep-chch-EnR | Wild-type | 48 | 48 | 0 | 41 | 6 | 1 | 0 | 0 |
LacZ | Wild-type | 26 | 26 | 0 | 23 | 3 | 0 | 0 | 0 |
chch-EnR/xFsast1-EnR | Wild-type | 38 | 38 | 0 | 26 | 6 | 0 | 0 | 6 |