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Table 3 Transplantation of chch-inhibited cells undergo inappropriate movements during gastrulation and results in cells in the mesoderm.

From: Churchill regulates cell movement and mesoderm specification by repressing Nodal signaling

   

Location of cells at 24 hpf

Location of cells at shield stage

Injected mRNA

Nb

Neural tissue

Epidermis

Notochord

Anterior somites

Posterior somites

Shield

LacZ

0

0

0

0

0

0

 

chch-EnR

10

2

0

6

2

0

 

chch-EnR/chch-mRNA

0

0

0

0

0

0

 

chch-ATGMO

1

0

0

1

0

0

Lateral Mesoderm

LacZ

1

0

0

0

1

0

 

chch-EnR

20

2

0

4

12

2

 

chch-EnR/chch-mRNA

2

0

0

0

2

0

 

chch-ATGMO

5

0

0

0

2

3

Ventral Mesoderm

LacZ

0

0

0

0

0

0

 

chch-EnR

14

1

1

0

10

2

 

chch-EnR/chch-mRNA

3

0

0

0

2

1

 

chch-ATGMO

2

0

0

0

1

1

  1. b-Same embryos were scored at 40% epiboly, shield stage and 24 hrs