Figure 1

Reactivity of the hybridomas GCTM-2 and TG30 used to fractionate hESC by flow cytometry. A, Protein lysate from M15 cells transfected (T) with the full-length human podocalyxin construct, pcDNA3/Podxl, shows immunoreactivity to podocalyxin antibodies (PHM5). In contrast, no immunoreactivity was observed with GCTM-2, TG343 or TRA 1–60 antibodies, nor in the untransfected (UT) cells. B, Indirect immunofluorescent staining of hESC for podocalyxin (green) and GCTM-2 (red). C, Human kidney stained for GCTM-2. D, Human kidney stained for podocalyxin. E, Mouse STO cells transfected with human CD9 cDNA stained by indirect immunofluorescence for CD9 (red) and TG30 (green) and DAPI (blue). CD9 and TG30 staining is entirely coincident. Bar in B-E = 100 μm.