Table 1 Primers used for real-time gene expression analysis. The amplicon size generated by each primer pair is shown.
Target | Primers | Fragment size (bp) | |
|---|---|---|---|
Primer pair name | Sequence | ||
sseKer1 | SseK1•1 | 5'-AAATCCAGAACCGCTACGCCATGC-3' (F) | 70 |
SseK1•2 | 5'-AGCTGCTCCTCCATACCGCTCACC-3' (R) | ||
sseKer2 | SseK2•1 | 5'-CGTCGCCTCCAAGAACCGCAGA-3' (F) | 86 |
SseK2•2 | 5'-CTGTGCTCACGGCCACCTCCTTA-3' (R) | ||
GAPDH2 | SseGAPDH231•1 | 5'-AGCCACCGTGTCGCCGACCT-3' (F) | 107 |
SseGAPDH231•2 | 5'-AAAAGAGGAGATGGTGGGGGGTGGT-3' (R) | ||
18S rRNA | Sse18S•1 | 5'-GAATTGACGGAAGGGCACCACCAG-3' (F) | 148 |
Sse18S•2 | 5'-ACTAAGAACGGCCATGCACCACCAC-3' (R) | ||