Figure 5

Rbp4 MO affects the early events of liver patterning prior to formation of liver bud. (A-F) foxa3 expression in both control (A-C) and rbp4 morphant (D-F) at 11 hpf (A, D), 16 hpf (B, E) and 25 hpf (C, F) respectively. In (B) and (E), triangles indicate the hatching gland and asterisks the tail bud. The arrows in (E) indicate the ectopic foxa3 expression in rbp4 morphant. The arrows in (C, F) indicate the liver buds. (G, H) Dorsal view of prox1 expression at 28 hpf in whole mount control (G) and morphant (H) embryos. (I, J) Cross section of the embryos as indicated by the dashed lines in (G, H) respectively. The point/dash lines represent midline of the embryos. (K-N), fn1 expression at 16 hpf in control (K, M) and rbp4 morphant (L, N): front view (K, L) and ventral view (M, N). The arrows in K and L indicate the fn1 expression in anterior lateral plate mesoderm.