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Figure 2 | BMC Developmental Biology

Figure 2

From: Common cellular events occur during wound healing and organ regeneration in the sea cucumber Holothuria glaberrima

Figure 2

Stages in wound healing following transection of H. glaberrima bodywall. Longitudinal tissue sections of injured body wall at (A) 2, (B) 6, (C) 12, (D) 20 and (E) 28 days post injury (dpi) were stained with Toluidene Blue. (A) At 2 dpi the area is filled with debris and a clot has been formed. (B) By 6 dpi, the wound area is filled with a transitory matrix that appears to contain some of the clot material as well as new extracellular material. The muscle has healed and the stumps appear to be regenerating. (C) By 12 dpi the bodywall connective tissue has recovered some of its original organization, although its composition appears different from the non-injured tissue. The longitudinal muscle stumps have grown but have not rejoined. (D) By 20 dpi the connective tissue still appears slightly disorganized but the muscle stumps have connected forming a continuous longitudinal muscle. (E) At 28 dpi, both the muscle and the connective tissue have recovered much of the structure and organization found within non-injured tissue. CM-circular muscle, D-dermis, LM-longitudinal muscle, LMS-longitudinal muscle stump, RN-radial nerve. X's denote the injury site; Asterisks show the presence of tattoo ink used to label the injury site; arrowheads signal the ampulla structures within the bodywall. Bar = 300 μm.

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