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Table 1 Mutant and morphants examined in the present study. Larval stages and number of specimens analysed are detailed in the table, together with a brief description of the overall phenotype and the morphology of the intraocular vasculature

From: Genetic determinants of hyaloid and retinal vasculature in zebrafish

NAME

CATEGORY

AGE

n

GENERAL PHENOTYPE

HYALOID -RETINAL PHENOTYPE

MAGP1

MORPHANT Microfibril

associated glycoprotein 1

3 dpf

15

Dilated brain and caudal vessels.

Irregular lumen of axial vasculature(Chen et al. 2006)

Stagnated growth of hyaloid vasculature

with less and thicker branches.

Aggregation of vascular endothelial

cells at the posterior lens

  

5 dpf

15

  

HS6ST2

MORPHANT heparan sulfate

sulfotransferase 2

5 dpf

15

Over-lumenized vessels and defective

branching in caudal vein plexus

(Chen et al. 2005)

Hyaloid vasculature with scarce and

oversized branches that display

an aberrant patterning.

Syn 2

MORPHANT Morphant

of syndecan 2

5 dpf

6

Aberrant or absent sprouting

in the intersegmental vessels

(Chen et al. 2004)

Sparse disorganized endothelial cells

surrounding very small lens.

Sppl2b

MORPHANT Signal peptide

peptidase like protein 2b

5 dpf

10

Erythrocyte accumulation in an

enlarged caudal vein

(Krawitz et al. 2005)

NO PHENOTYPE

Mab21l2

MORPHANT Male abnormal

21 like-2 protein

5 dpf

5

Microphthalmia. Apoptosis in the

developing retina and lens

(Kennedy et al. 2004)

Thicker vessels that cover

only posterior part

of the lens.

obd

VASCULAR MUTANT Plexin-D1

out of bounds

3 dpf

5

Mispatterning of the trunk intersegmental

vessels (variable severity).

~10% of mutants die before 6 dpf

(Childs et al. 2002; Torres-Vazquez et al. 2004)

Mis-patterning of the hyaloid vessels

(variable severity). Abnormal branching and extra

interconnections obvious at 5 dpf.

Atypical loops, tortuosity and increased

number of vessels in the

retinal vasculature of the adult.

  

5 dpf

20

  
  

6 dpf

5

  
  

10 dpf

5

  
  

20 dpf

3

  
  

Adult

10

  

ace

VASCULAR MUTANT

FGF8 Hypomorph

acerebellar

5 dpf

5

General CNS abnormalities.

Absence of vessels in the dorsal brain.

Abnormal heart development

(Reifers et al. 1998; Reifers et al. 2000)

NO PHENOTYPE

arl

LENS MUTANT Laminin alpha1

arrested lens

3 dpf

3

Abnormal eye development.

Lens development halted at 24 hpf.

Lack of any lens structure from 48 hpf.

(Vihtelic et al. 2001;

Vihtelic and Hyde 2002;

Semina et al. 2006)

Complete absence of

hyaloid vessels in all

stages analysed

  

4 dpf

3

  
  

5 dpf

3

  
  

6 dpf

3

  

mgf

LENS MUTANT/Unknown

margin affected

4 dpf

7

Abnormal eye development from 3 dpf.

Very small lens

(Unpublished)

At 4 dpf rudimentary hyaloid vasculature

(fewer vessels and branches) on lens.

At 5 dpf complete absence

of hyaloid vasculature on small lens

  

5 dpf

8

  

dsl

LENS MUTANT/Unknown

disrupted lens

5 dpf

5

Abnormal eye and lens development.

(Vihtelic et al. 2001;

Vihtelic and Hyde 2002)

NO PHENOTYPE

  

6 dpf

4

  
  

8 dpf

5

  

fe

LENS-VASCULAR MUTANT/Unknown

fused eyes

3 dpf

7

Synophthalmia. Abnormal/absent

intersegmental blood vessels.

(Unpublished)

No hyaloid vessels

on the lens

plt

LENS MUTANT/Unknown

platinum

4 dpf

3

Defects in melanin pigmentation.

Photoreceptor and RPE degeneration from 5 dpf.

(Vihtelic et al. 2001;

Vihtelic and Hyde 2002)

Premature detachment of

hyaloid vessels from the lens at 4 dpf.

Less vessels and less patterning

of the branches covering only

posterior lens at 6 dpf.

  

5 dpf

3

  
  

6 dpf

3

  

lop

LENS MUTANT/Unknown

lens opaque

6 dpf

4

Lens opacity

(Vihtelic et al. 2005)

NO PHENOTYPE