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Table 1 Mutant and morphants examined in the present study. Larval stages and number of specimens analysed are detailed in the table, together with a brief description of the overall phenotype and the morphology of the intraocular vasculature

From: Genetic determinants of hyaloid and retinal vasculature in zebrafish

NAME CATEGORY AGE n GENERAL PHENOTYPE HYALOID -RETINAL PHENOTYPE
MAGP1 MORPHANT Microfibril
associated glycoprotein 1
3 dpf 15 Dilated brain and caudal vessels.
Irregular lumen of axial vasculature(Chen et al. 2006)
Stagnated growth of hyaloid vasculature
with less and thicker branches.
Aggregation of vascular endothelial
cells at the posterior lens
   5 dpf 15   
HS6ST2 MORPHANT heparan sulfate
sulfotransferase 2
5 dpf 15 Over-lumenized vessels and defective
branching in caudal vein plexus
(Chen et al. 2005)
Hyaloid vasculature with scarce and
oversized branches that display
an aberrant patterning.
Syn 2 MORPHANT Morphant
of syndecan 2
5 dpf 6 Aberrant or absent sprouting
in the intersegmental vessels
(Chen et al. 2004)
Sparse disorganized endothelial cells
surrounding very small lens.
Sppl2b MORPHANT Signal peptide
peptidase like protein 2b
5 dpf 10 Erythrocyte accumulation in an
enlarged caudal vein
(Krawitz et al. 2005)
NO PHENOTYPE
Mab21l2 MORPHANT Male abnormal
21 like-2 protein
5 dpf 5 Microphthalmia. Apoptosis in the
developing retina and lens
(Kennedy et al. 2004)
Thicker vessels that cover
only posterior part
of the lens.
obd VASCULAR MUTANT Plexin-D1
out of bounds
3 dpf 5 Mispatterning of the trunk intersegmental
vessels (variable severity).
~10% of mutants die before 6 dpf
(Childs et al. 2002; Torres-Vazquez et al. 2004)
Mis-patterning of the hyaloid vessels
(variable severity). Abnormal branching and extra
interconnections obvious at 5 dpf.
Atypical loops, tortuosity and increased
number of vessels in the
retinal vasculature of the adult.
   5 dpf 20   
   6 dpf 5   
   10 dpf 5   
   20 dpf 3   
   Adult 10   
ace VASCULAR MUTANT
FGF8 Hypomorph
acerebellar
5 dpf 5 General CNS abnormalities.
Absence of vessels in the dorsal brain.
Abnormal heart development
(Reifers et al. 1998; Reifers et al. 2000)
NO PHENOTYPE
arl LENS MUTANT Laminin alpha1
arrested lens
3 dpf 3 Abnormal eye development.
Lens development halted at 24 hpf.
Lack of any lens structure from 48 hpf.
(Vihtelic et al. 2001;
Vihtelic and Hyde 2002;
Semina et al. 2006)
Complete absence of
hyaloid vessels in all
stages analysed
   4 dpf 3   
   5 dpf 3   
   6 dpf 3   
mgf LENS MUTANT/Unknown
margin affected
4 dpf 7 Abnormal eye development from 3 dpf.
Very small lens
(Unpublished)
At 4 dpf rudimentary hyaloid vasculature
(fewer vessels and branches) on lens.
At 5 dpf complete absence
of hyaloid vasculature on small lens
   5 dpf 8   
dsl LENS MUTANT/Unknown
disrupted lens
5 dpf 5 Abnormal eye and lens development.
(Vihtelic et al. 2001;
Vihtelic and Hyde 2002)
NO PHENOTYPE
   6 dpf 4   
   8 dpf 5   
fe LENS-VASCULAR MUTANT/Unknown
fused eyes
3 dpf 7 Synophthalmia. Abnormal/absent
intersegmental blood vessels.
(Unpublished)
No hyaloid vessels
on the lens
plt LENS MUTANT/Unknown
platinum
4 dpf 3 Defects in melanin pigmentation.
Photoreceptor and RPE degeneration from 5 dpf.
(Vihtelic et al. 2001;
Vihtelic and Hyde 2002)
Premature detachment of
hyaloid vessels from the lens at 4 dpf.
Less vessels and less patterning
of the branches covering only
posterior lens at 6 dpf.
   5 dpf 3   
   6 dpf 3   
lop LENS MUTANT/Unknown
lens opaque
6 dpf 4 Lens opacity
(Vihtelic et al. 2005)
NO PHENOTYPE