Figure 4
From: Sex-specific DoublesexM expression in subsets of Drosophilasomatic gonad cells
DSXM but not TJ is expressed in male-specific somatic gonadal precursors. (A-D) Stage 13 testis immunofluorescence using (A) anti-DSXM, (B) anti-EYA, and (C) anti-VASA. (D) Merged images A-C. A DSXM and EYA positive cluster of cell nuclei is located posterior and ventral to the other cells of the somatic gonad (arrows). (E-H) Stage 13 male testis immunofluorescence using (E) anti-DSXM, (F) anti-SOX100B and (G) anti-VASA. (H) Merged images E-G. (I-L) Stage 15 testis immunofluorescence using (I) anti-DSXM, (J) anti-SOX100B, and (K) anti-VASA antibody. (L) Merged images I-K. (M-P) Stage 15 testis immunofluorescence using (M) anti-TJ, (N) anti-SOX100B and (O) anti-VASA. (P) Merged images M-O. The scale bars = 10 μm. Anterior is to the left. Secondary antibodies were: (A, E, I) biotin-coupled goat anti-rat and TSA, (B) Alexa 488 goat anti-mouse, (C) Cy5 goat anti-rabbit), (F, J) Alexa 488 goat anti-rabbit, (G, K, O) Alexa 647 goat anti-chicken, (M) Alexa 488 goat anti-guinea pig, (N) biotin-coupled goat anti-rabbit and TSA.