Figure 3
From: Sex-specific DoublesexM expression in subsets of Drosophilasomatic gonad cells
DSXM and TJ expression in the male somatic gonad. (A-H) Stage 13 male embryo immunofluorescence using: (A) anti-DSXM, (B) anti-TJ, and (C) anti-VASA. (D) Merged images A-C. (E-H) Magnified view of the gonad in A-D. Somatic nuclei expressing DSXM but not TJ are indicated (arrows). (I-P) Stage 15 male embryo immunofluorescence using: (I) anti-DSXM, (J) anti-TJ, and (K) anti-VASA. (L) Merged images I-K. (M-P) Magnified view of the gonad in I-L. The scale bars = 50 μm in A-D; I-L and 10 μm in E-H; M-P. Anterior is to the left. Secondary antibodies were: (A, I) biotin-coupled goat anti-rat with TSA, (B, J) Alexa 488 goat anti-guinea pig, and (C, K) Cy5 goat anti-rabbit.