Figure 5

Reduction of apoptotic cells in LIF treated sphere-derived cell cultures 48 h after plating. Annexin-positive cells and annexin & nestin-double positive cell populations were significantly decreased in response to LIF. Error bars indicate S.E.M., * indicates p < 0.05, ** indicates p < 0.01, n = 4. Total cell numbers did not differ significantly when we compared LIF-treated with untreated cultures (1124 ± 363 and 894 ± 516, respectively).