Figure 3From: LIF promotes neurogenesis and maintains neural precursors in cell populations derived from spiral ganglion stem cellsIncreased BrdU incorporation and increased number of neural progenitor cells in response to LIF treatment. (A): Quantification of BrdU-positive, nestin-positive, and BrdU/nestin-double positive cells 48 h after plating. Error bars indicate S.E.M., * indicates p < 0.05, ** indicates p < 0.01, n = 8. Total cell numbers did not differ significantly when we compared LIF-treated with untreated cultures (1934 ± 403 and 2008 ± 435, respectively). (B-C): Representative pictures of plated sphere-derived cells without LIF treatment (B), and with 10 ng/ml LIF (C). Scale bar = 200 μm. (C'-C''): Higher magnification of two nestin-positive cells from (C) to illustrate the nuclear localization of the BrdU staining in one of the cells.Back to article page