LIF treatment leads to an increase of TuJ and GFAP positive cells without affecting the total cell number. (A): The total cell number of sphere-derived cells after 10 days in culture did not significantly change when treated with different concentrations of LIF. n = 4. (B): The percentage of TuJ-positive cells was significantly increased in a dose-dependent manner up-to 7-fold. Likewise, the percentage of GFAP-expressing cells was increased in response to LIF. The percent values express the fraction of immunopositive cells of the total cell number. Error bars indicate S.E.M. * indicates p < 0.05, ** indicates p < 0.01, n = 6. (C-E): Representative images of differentiated cells of non-LIF-treated cultures (C), and cultures treated with 0.1 ng/ml (D) and 10 ng/ml LIF (E). Scale bar = 200 μm.