Figure 1From: LIF promotes neurogenesis and maintains neural precursors in cell populations derived from spiral ganglion stem cellsLIF treatment leads to an increase of TuJ and GFAP positive cells without affecting the total cell number. (A): The total cell number of sphere-derived cells after 10 days in culture did not significantly change when treated with different concentrations of LIF. n = 4. (B): The percentage of TuJ-positive cells was significantly increased in a dose-dependent manner up-to 7-fold. Likewise, the percentage of GFAP-expressing cells was increased in response to LIF. The percent values express the fraction of immunopositive cells of the total cell number. Error bars indicate S.E.M. * indicates p < 0.05, ** indicates p < 0.01, n = 6. (C-E): Representative images of differentiated cells of non-LIF-treated cultures (C), and cultures treated with 0.1 ng/ml (D) and 10 ng/ml LIF (E). Scale bar = 200 μm.Back to article page