Ptf1a misexpression differentially affects amacrine cell subtypes genesis. Quantification of GABA-, serotonin (5-HT)-, tyrosine hydroxylase (TH)- and glycine-positive cells, following Ptf1a Mo (stage 40/41) or Ptf1a-GR (stage 39, except in O, P, R stage 41) injection in two cell stage embryos. (A-D, G-J, M-P, S-V) Typical sections of control, Ptf1a knocked-down or Ptf1a overexpressing retinas, immunostained for GABA, 5-HT, TH or glycine (arrows) as indicated. Graphs indicate the average numbers of GABA-, 5-TH-, TH- or glycine-positive cells per retinal section in each condition. Values are given as mean +/- s.e.m. p < 0.001 (***), p < 0.05 (*) (Student's t test). Ctrl: control; L: lens. Scale Bar represents 50 μm.