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Figure 5 | BMC Developmental Biology

Figure 5

From: Term amniotic membrane is a high throughput source for multipotent mesenchymal stem cells with the ability to differentiate into endothelial cells in vitro

Figure 5

AM-hMSCs multi-lineage differentiations in vitro : chondrogenic (A), osteogenic (B), adipogenic (C-H) commitments. Chondrogenic differentiation revealed by immunohistochemical stain for collagen II in induced AM-hMSCs. Original magnification ×40 (A). Osteogenic differentiation evidenced by the formation of mineralized matrix as shown by von Kossa staining. Original magnification ×10 (B).Small colonies with lipid secretion during the first week of adipogenic induction as highlighted by Red Oil staining for neutral lipids. Magnification ×4 (C); at higher magnification, multivacuolar adipogenic cells. Magnification ×10 and ×25 respectively (D, E). Big aggregates with intensive and massive lipid secretion at the third week of adipogenic induction. Magnification ×10, ×10 and ×40 respectively (F, G, H).

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