Table 3 Information on primers used for qPCR
Gene/EST | Source | Primer Sequence | Ta (°C) | Amplicon size (bp) |
|---|---|---|---|---|
ACTB 1 | AJ312193 | GAGAAGCTCTGCTACGTCGC | 59 | 231 |
CCTGATGTCCACGTCGCACT | ||||
POLR2A 1 | DT324622 | GAAGGGGGAGAGACAAACTG | 60 | 86 |
GGGAGGAAGAAGAAAAAGGG | ||||
RPL32 1 | NM_001001636 | AGCCCAAGATCGTCAAAAAG | 55 | 165 |
TGTTGCTCCCATAACCAATG | ||||
AGPAT1 1 | AL773562 | AGGACGCAACGTCGAGAACA | 60 | 110 |
GTGAGGGAGGGAAGTGGTGAG | ||||
CANX 1 | AJ653783 | CAATGATGGATGGGGTCTGAA | 60 | 135 |
AACACAGGTAATGCCACAGTCAA | ||||
NME12 | EH792596 | TGTGGAGAGCGCAGAGAAAGA | 58 | 143 |
GGGAGAGAGGAGAAATGGAATGG | ||||
IGSF1 2 | EH792632 | GAGTCCACCCCATCTACTGTTCC | 60 | 60 |
AAATCCCCTTGACCCATCTCA | ||||
HMGA2 2 | EH792601 | AAGGAGGCAGAAGCAGAATGA | 59 | 74 |
TGGAGACCCTCAGAGACAAGAA | ||||
GATA3 3 | EH792592 | CTTAGGGAAGATGAGTCTGAATGG | 59 | 125 |
TTTTGAAGGCAGAAAGCGAAG | ||||
SPP1 3 | X16575 | TTGCTAAAGCCTGACCCATCT | 60 | 145 |
CGTCGTCCACATCGTCTGTT | ||||
SMC6L1 3 | EH792668 | TGAGGCAGTATCAAGAAGCAAAAG | 58 | 189 |
AACAACAGCAACAAAAGAGCCAA | ||||
RAB6IP2 3 | EH792612 | GGATGTGAAGGAGCGGAAAG | 60 | 121 |
AGCCTGCAATGATTTGACTCG | ||||
PDGFRA 3 | EH792663 | CAGGCAGGTTGGAGGGAGAT | 60 | 101 |
AAGTTGCGGAGGTTGGATTCT | ||||
TJP1 3 | EH792652 | CTGGGCTCTTGGCTTGCTATTC | 60 | 130 |
CTCCTCCTGCCGTTTTTGG | ||||
LXN 3 | CB477731 | CAAGCAAGTGCAAAGAAATGATG | 60 | 151 |
TGGCAGACGGCTGTTATGTT | ||||
NRAP 3 | EH792618 | AAGTGAGGCAGTCTCCAGAGG | 60 | 86 |
CATATCCCAGTGAAACACCGAT | ||||
qR15C1 3 | Ti784825315 | ACAGTGAGAGCGAGCGTGATG | 60 | 158 |
TGCTTTCCCTTTATCGGAGG | ||||
bR10D1 3 | DQ631863 | GCTACACATTCAGCACAGAGTAAGA | 60 | 126 |
CTGGGGAAAGACTCCAAAAGA |