Figure 4

Some distinct features of UAS-ara overexpressing cells. UAS-ara expressing cells (green) were detected by coexpression of UAS-GFP, except in F and G where they were labelled with an anti Ara/Caup antibody. Counterstaining (in red) was Actin (B-D, F, G) or E-Cadherin (E). (A, A') UAS-ara-expressing cells distort the prospective wing margin (anti Cut staining, purple) by apparently pulling on it (arrowheads). (B, B') Apical x, y section of a connecting stripe between larger masses of UAS-ara expressing cells. Cell contours (arrowheads) are stretched parallel to the longitudinal axis of the stripe. (C, D) xy and z optical sections, respectively, of a small group of wild-type cells surrounded by UAS-ara-expressing cells. The former undergo apical-basal shortening (arrowheads). (E) z section of a group of wild-type cells surrounded by UAS-ara expressing cells. The apical marker DE-Cadherin shows that a fold has been formed at the interface (arrowheads). (F, G) xy and z optical sections, respectively, of UAS-ara expressing clones that have induced folds of the wing pouch epithelium (arrowheads) that comprise overexpressing and non-expressing cells. The folds can join with the extant folds of the disc (arrow). Dashes mark the approximate plane of the reciprocal view. For orientation, blue arrows in D, E and G point towards the apical part of cells.