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Figure 4 | BMC Developmental Biology

Figure 4

From: Gene delivery into mouse retinal ganglion cells by in utero electroporation

Figure 4

The timing of electroporation affects the area of gene targeting. (A) In flattened whole mount E16 retinas electroporated with GFP-plasmids at E13, the cells in the central retina are targeted (green cells). In contrast, when electroporation is performed at E14 the location of GFP-expressing cells varies depending on the position of the electrodes. Examples of retinas electroporated at E14 in the medial dorso-nasal region (B) medial ventronasal (C) or the very peripheral ventronasal retina (D). d, dorsal; n, nasal; t, temporal; v, ventral. Scale bar: 500 μm.

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