Figure 3
From: Alpha6beta1 integrin expressed by sperm is determinant in mouse fertilization
Flow cytometric analysis of alpha6beta1 integrin expression on mouse sperm. Expression of α6 and β1 integrin subunits on capacitated mouse sperm was evaluated by flow cytometry. Sperm cells were incubated with the mAb anti-α6 (GoH3) (A) or anti-β1 (MB1.2) (B) or Rat IgG2a isotype negative control (A, B), stained with Alexa Fluor® 594 (red) goat anti-rat IgG, then analyzed by flow cytometry on the basis of FSC/FL1 (cell size/relative fluorescence) parameters.