Figure 2
From: Alpha6beta1 integrin expressed by sperm is determinant in mouse fertilization
Expression and distribution of alpha6beta1 integrin on mouse sperm. Distribution of α6β1 integrin was studied by immunofluorescence microscopy. Spermatozoa were exposed to FITC-PSA (a, d, g, j) after anti-α6 integrin GoH3 antibody (b, e, h, k) or anti-β1 integrin MB1.2 antibody (e', h', k') followed by Alexa Fluor® 594 (red) goat anti-rat IgG secondary antibody. The following fluorescent patterns were recorded: negative non capacitated sperm (b), equatorial localization on capacitated sperm (e, e', h, h') and post-acrosomal localization (k, k') only on capacitated AR sperm. c, f, i and l represent composite images generated by superimposition of the green and red signals. Arrows indicate the position of intact (white) or reacted (yellow) acrosome. In order to distinguish the different spermatic regions, they were delimited on the image (a); 1: acrosome, 2: equatorial region and 3: post-acrosomal region. Bar = 5 μm.