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Table 1 p75 NTR and Hu C/D Expression in Acutely Isolated Cells from St. 29/30 (E6.5) Sympathetic Ganglia

From: Embryonic sympathoblasts transiently express TrkB in vivo and proliferate in response to brain-derived neurotrophic factor in vitro

 

Experiment 1 (mean values; n = 3)

Experiment 2 (mean values; n = 3)

p75NTR (% of total)

1974.3 +/- 368 (91%)

2128.7 +/- 490 (80%)

Hu C/D (% of total)

719.7 +/- 137 (33%)

751 +/- 47 (28%)

Total Number

2177.7 +/- 381

2637 +/- 701

  1. Cells isolated from dispersed St 29/30 (E6.5) lumbar sympathetic ganglia were plated onto poly-lysine and laminin coated cover slips and allowed to attach for 2 hrs. Cover slips were then fixed and stained for p75 NTR and Hu C/D using double immunofluorescence. P75 NTR marks all cells derived from the neural crest, regardless of their state of differentiation. Hu C/D is a neural-specific RNA binding protein that is an early marker of neural differentiation. Each experiment is a separate plating and 3 cover slips were counted for each experiment.
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BMC Developmental Biology

ISSN: 1471-213X

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