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Figure 3 | BMC Developmental Biology

Figure 3

From: A high throughput messenger RNA differential display screen identifies discrete domains of gene expression and novel patterning processes along the developing neural tube

Figure 3

Differential display gel profile and corresponding chick embryo whole mount in situ hybridization. (A, A') Clone A (250 bp; Engrailed 1). (B, B') Clone D (700 bp; Sprouty 2). (C, C') Clone 2 (560 bp; RTPzeta). (D, D') Clone β (440 bp; Pax 2: genome-derived identity). (E, E') Clone G (406 bp; Unknown). (F, F') Clone L (220 bp; L1CAM). (G, G') Clone 5 (450 bp; FGFR2) (H, H') Clone C (525 bp; similar to SPARC related protein 1: genome-derived identity). (I, I') Clone 3 (660 bp; Unknown). (J, J') Clone 4 (257 bp; EF1α). (K, K') Clone F (586 bp; similar to HB-GAM: genome-derived identity). (L, L') Clone B (600 bp; MafB). (M, M') Clone Q (1700 bp; LRP37/p40) (N, N') Clone E (1200 bp; KIAA0007). In all cases the observed expression pattern correlates with that expected from the differential display gel profile. F = forebrain, M = midbrain, R1 = rhombomere 1, H = hindbrain (rhombomeres 2–7). The arrow (black or white) denotes the position of the differentially expressed PCR product. The size of the original PCR product is given in base pairs (bp). See also Table 1.

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BMC Developmental Biology

ISSN: 1471-213X

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