Figure 2

Overexpression of XDab2 disrupts the sprouting of ISV. (A and B) Injection of XDab2 RNA inhibits the formation of the sprouting ISV on the injected side of the embryo, with that on the uninjected side being normal. The same amount of control nuclear β-galactosidase (β-gal) RNA shows no effects on the ISV sprouts. One blastomere of two-cell stage embryos was injected with β-gal RNA as a lineage tracer with or without XDab2 RNA. Embryos were fixed at stage 34, stained for β-gal and then hybridized against Xmsr (A) or EphB4 (B). Arrows and arrowheads indicate the normal and disrupted ISV on the injected side of the embryo, respectively. Rectangular areas in the upper panels are enlarged in the lower panels. (C) The table showing the results from the gain-of-function analysis of XDab2. Overexpression of XDab2 causes the angiogenic defects in a dose-dependent manner.