Figure 7
From: Implantation Serine Proteinases heterodimerize and are critical in hatching and implantation
Effect of inhibitors and antibodies on hatching and invasion. In vitro hatching and outgrowth of blastocysts when grown in culture medium supplemented with different concentrations of Gabexate mesylate, anti-ISP1/-ISP2 mAbs (blue: embryos per set; purple: hatched after 24 h/outgrown after 48 h; white: hatched after 48 h/outgrown after 72 h). A – Effect of Gabexate on hatching (P value – 0.03). B – Effect of Gabexate on outgrowth (P value – 0.006). C – Effect of anti-ISP1 mAb on hatching (P value – 0.03). D – Effect of anti-ISP1 mAb on outgrowth (P value – not significant). E – Effect of anti ISP2 mAb on hatching of blastocysts (P value at 24 hrs. – 0.02; at 48 hrs. – not significant). F – Effect of anti-ISP2 mAb on the outgrowth of hatched blastocysts (P value – not significant). G – Effect of anti-ISP1/ISP2 mAb on the hatching of blastocysts (P value – 0.003). H – Effect of anti-ISP1/ISP2 mAb on the outgrowth of hatched blastocysts (P value – 0.006).