A role for Phospholipase D in Drosophilaembryonic cellularization

Table 1 Cellularization is delayed in homozygous Pld^nullembryos

Embryo	Total time from cellularization start to pole cell migration (min:sec)
yw #1	65:49
yw #2	68:09
yw #3	71:15
Embryo	Total time from cellularization start to pole cell migration (min:sec)	increase in time required for cellularization
Pld^null#1	69:42	1.9%
Pld^null#2	86:26	26.4%
Pld^null#3	69:30	1.6%
Pld^null#4	88:33	29.4%
Pld^null#5	75:12	9.9%
Pld^null#6	86:21	26.2%

The embryonic development of single embryos from the time of pole cell formation to the start of gastrulation was recorded using a stereomicroscope with an attached video recorder. The videos were analyzed from the start of cellularization (marked by initial membrane invagination and pole cell formation) to the beginning of pole cell migration and gastrulation. Pld^nullembryos were collected from cages set up with homozygous Pld^nullmales and females from an outcrossed heterozygous Pld^nullstock.

ISSN: 1471-213X